Toward the Second Generation of Optogenetic Tools
Name
Knopfel-2010-Toward the Second Generation of Optogenetic Tools.pdf
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1.35 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Knopfel, Thomas
Zin, Michael Z.
Levskaya, Anselm
Tian, Lin
Lin, John Y.
Boyden, Edward
Date Issued
November 2010
Journal
Journal of Neuroscience
Publisher
Society for Neuroscience
Citation
Knopfel, T. et al. “Toward the Second Generation of Optogenetic Tools.” Journal of Neuroscience 30.45 (2010) : 14998-15004.
Version
Final published version
Abstract
This mini-symposium aims to provide an integrated perspective on recent developments in optogenetics. Research in this emerging field combines optical methods with targeted expression of genetically encoded, protein-based probes to achieve experimental manipulation and measurement of neural systems with superior temporal and spatial resolution. The essential components of the optogenetic toolbox consist of two kinds of molecular devices: actuators and reporters, which respectively enable light-mediated control or monitoring of molecular processes. The first generation of genetically encoded calcium reporters, fluorescent proteins, and neural activators has already had a great impact on neuroscience. Now, a second generation of voltage reporters, neural silencers, and functionally extended fluorescent proteins hold great promise for continuing this revolution. In this review, we will evaluate and highlight the limitations of presently available optogenic tools and discuss where these technologies and their applications are headed in the future.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1523/jneurosci.4190-10.2010
