Biotinylated polyurethane-urea nanoparticles for targeted theranostics in human hepatocellular carcinoma
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12274_2014_Article_678.pdf
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Author(s)
Morral-Ruíz, Genoveva
López-Vicente, Andrea
Solans, Conxita
García-Celma, María José
Melgar Lesmes, Pedro
Date Issued
February 2015
Journal
Nano Research
Publisher
Tsinghua University Press
Citation
Morral-Ruíz, Genoveva, Pedro Melgar-Lesmes, Andrea López-Vicente, Conxita Solans, and María José García-Celma. “Biotinylated Polyurethane-Urea Nanoparticles for Targeted Theranostics in Human Hepatocellular Carcinoma.” Nano Research 8, no. 5 (February 4, 2015): 1729–1745.
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Author's final manuscript
Abstract
Over the past years, significant efforts have been devoted to explore novel drug delivery and detection strategies for simultaneous therapy and diagnostics. The development of biotinylated polyurethane-urea nanoparticles as theranostic nanocarriers for targeted drug and plasmid delivery, for fluorescence detection of human hepatocellular carcinoma cells, is described herein. These targeted nanoparticles are specifically designed to incorporate biotin into the polymeric matrix, since many tumor types overexpress receptors for biotin as a mechanism to boost uncontrolled cell growth. The obtained nanoparticles were spherical, exhibited an average diameter ranging 110–145 nm, and showed no cytotoxicity in healthy endothelial cells. Biotinylated nanoparticles are selectively incorporated into the perinuclear and nuclear area of the human hepatocellular carcinoma cell line, HepG2, in division, but not into growing, healthy, human endothelial cells. Indeed, the simultaneous incorporation of the anticancer drugs, phenoxodiol or sunitinib, together with plasmid DNA encoding green fluorescent protein, into these nanoparticles allows a targeted pharmacological antitumor effect and furthermore, selective transfection of a reporter gene, to detect these cancer cells. The combined targeted therapy and detection strategy described here could be exploited for liver cancer therapy and diagnostics, with a moderate safety profile, and may also be a potential tool for other types of cancer.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1007/s12274-014-0678-6
