Dosimetry of N[superscript 6]-Formyllysine Adducts Following [[superscript 13]C[superscript 2]H[subscript 2]]-Formaldehyde Exposures in Rats
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Edrissi_2013-Dosimetry of N6.pdf
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Author(s)
Edrissi, Bahar
Taghizadeh, Koli
Moeller, Benjamin C.
Kracko, Dean
Doyle-Eisele, Melanie
Swenberg, James A.
Dedon, Peter C.
Date Issued
October 2013
Journal
Chemical Research in Toxicology
Publisher
American Chemical Society (ACS)
Citation
Edrissi, Bahar, Koli Taghizadeh, Benjamin C. Moeller, Dean Kracko, Melanie Doyle-Eisele, James A. Swenberg, and Peter C. Dedon. “Dosimetry of N6-Formyllysine Adducts Following [13C2H2]-Formaldehyde Exposures in Rats.” Chemical Research in Toxicology 26, no. 10 (October 21, 2013): 1421-1423. © 2013 American Chemical Society
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Final published version
Abstract
With formaldehyde as the major source of endogenous N[superscript 6]-formyllysine protein adducts, we quantified endogenous and exogenous N[superscript 6]-formyllysine in the nasal epithelium of rats exposed by inhalation to 0.7, 2, 5.8, and 9.1 ppm [[superscript 13]C[superscript 2]H[subscript 2]]-formaldehyde using liquid chromatography-coupled tandem mass spectrometry. Exogenous N[superscript 6]-formyllysine was detected in the nasal epithelium, with concentration-dependent formation in total as well as fractionated (cytoplasmic, membrane, nuclear) proteins, but was not detected in the lung, liver, or bone marrow. Endogenous adducts dominated at all exposure conditions, with a 6 h 9.1 ppm formaldehyde exposure resulting in one-third of the total load of N[superscript 6]-formyllysine being derived from exogenous sources. The results parallel previous studies of formaldehyde-induced DNA adducts.
MIT Department
Massachusetts Institute of Technology. Center for Environmental Health Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1021/tx400320u
