Biased migration of confined neutrophil-like cells in asymmetric hydraulic environments
Name
Prentice-Mott-2013-Biased migration of.pdf
Size
1.03 MB
Format
Adobe PDF
Checksum (MD5)
c853f39a3178950b84d14fccb5a37e6e
Author(s)
Mahadevan, L.
Prentice-Mott, Harrison V.
Chang, Chi-Han
Mitchison, Timothy J.
Irimia, Daniel
Shah, Jagesh Vijaykumar
Date Issued
December 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Prentice-Mott, H. V., C.-H. Chang, L. Mahadevan, T. J. Mitchison, D. Irimia, and J. V. Shah. “Biased Migration of Confined Neutrophil-Like Cells in Asymmetric Hydraulic Environments.” Proceedings of the National Academy of Sciences 110, no. 52 (December 24, 2013): 21006–21011.
Version
Final published version
Abstract
Cells integrate multiple measurement modalities to navigate their environment. Soluble and substrate-bound chemical gradients and physical cues have all been shown to influence cell orientation and migration. Here we investigate the role of asymmetric hydraulic pressure in directional sensing. Cells confined in microchannels identified and chose a path of lower hydraulic resistance in the absence of chemical cues. In a bifurcating channel with asymmetric hydraulic resistances, this choice was preceded by the elaboration of two leading edges with a faster extension rate along the lower resistance channel. Retraction of the “losing” edge appeared to precipitate a final choice of direction. The pressure differences altering leading edge protrusion rates were small, suggesting weak force generation by leading edges. The response to the physical asymmetry was able to override a dynamically generated chemical cue. Motile cells may use this bias as a result of hydraulic resistance, or “barotaxis,” in concert with chemotaxis to navigate complex environments.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.1317441110
