Invasive breast carcinoma cells from patients exhibit Mena[superscript INV]- and macrophage-dependent transendothelial migration
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Gertler_Invasive breast.pdf
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Author(s)
Gertler, Frank
Pignatelli, Jeanine
Goswami, Sumanta
Jones, Joan G.
Rohan, Thomas E.
Pieri, Evan
Chen, Xiaoming
Adler, Esther
Cox, Dianne
Maleki, Sara
Date Issued
November 2014
Journal
Science Signaling
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Pignatelli, J., S. Goswami, J. G. Jones, T. E. Rohan, E. Pieri, X. Chen, E. Adler, et al. “Invasive Breast Carcinoma Cells from Patients Exhibit Mena[superscript INV]- and Macrophage-Dependent Transendothelial Migration.” Science Signaling 7, no. 353 (November 25, 2014): ra112–ra112. © 2014 American Association for the Advancement of Science
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Author's final manuscript
Abstract
Metastasis is a complex, multistep process of cancer progression that has few treatment options. A critical event is the invasion of cancer cells into blood vessels (intravasation), through which cancer cells disseminate to distant organs. Breast cancer cells with increased abundance of Mena [an epidermal growth factor (EGF)–responsive cell migration protein] are present with macrophages at sites of intravasation, called TMEM sites (for tumor microenvironment of metastasis), in patient tumor samples. Furthermore, the density of these intravasation sites correlates with metastatic risk in patients. We found that intravasation of breast cancer cells may be prevented by blocking the signaling between cancer cells and macrophages. We obtained invasive breast ductal carcinoma cells of various subtypes by fine-needle aspiration (FNA) biopsies from patients and found that, in an in vitro transendothelial migration assay, cells that migrated through a layer of human endothelial cells were enriched for the transcript encoding Mena[superscript INV], an invasive isoform of Mena. This enhanced transendothelial migration required macrophages and occurred with all of the breast cancer subtypes. Using mouse macrophages and the human cancer cells from the FNAs, we identified paracrine and autocrine activation of colony-stimulating factor-1 receptor (CSF-1R). The paracrine or autocrine nature of the signal depended on the breast cancer cell subtype. Knocking down Mena[superscript INV] or adding an antibody that blocks CSF-1R function prevented transendothelial migration. Our findings indicate that Mena[superscript INV] and TMEM frequency are correlated prognostic markers and CSF-1 and Mena[superscript INV] may be therapeutic targets to prevent metastasis of multiple breast cancer subtypes.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1126/scisignal.2005329
