PCR - Ligation Assembly Standard for BioBrick Parts
Name
BBFRFC83.pdf
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613.55 KB
Format
Adobe PDF
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Author(s) • •
He, Tony PeiYuan
Campbell, Alexander
Zhou, Stefanie Yifan
Date Issued
December 15, 2011
Series/Report no.
BBF RFC;83
Abstract
This Request for Comments (RFC) describes a novel method for the assembly of standard BioBrick parts. This assembly method for BioBrick parts is an improvement upon the conventional methods of BioBrick part assembly. This assembly method will involve the use of standard BioBrick restriction enzyme systems, PCR purification kit, ligase system, and a PCR polymerase system.
Subjects
DNA assembly
Terms of Use
Attribution-NoDerivs 3.0 United States
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