Three-dimensional differential interference contrast microscopy using synthetic aperture imaging
Name
Kim-2012-Three-dimensional di.pdf
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3.27 MB
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Checksum (MD5)
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Author(s)
Dasari, Ramachandra Rao
Feld, Michael S.
Sung, Yongjin
Kim, Moonseok
Choi, Youngwoon
Fang-Yen, Chris
Kim, Kwanhyung
Choi, Wonshik
Date Issued
February 2012
Journal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Kim, Moonseok et al. “Three-dimensional Differential Interference Contrast Microscopy Using Synthetic Aperture Imaging.” Journal of Biomedical Optics 17.2 (2012): 026003. © 2012 SPIE
Version
Final published version
Abstract
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.
MIT Department
Massachusetts Institute of Technology. Spectroscopy Laboratory
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/1.jbo.17.2.026003
