Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography
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Liu-2013-Method for Quantitat.pdf
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Author(s)
Diephuis, Bradford J.
Tearney, Guillermo J.
Liu, Linbo
Chu, Kengyeh K.
Houser, Grace H.
Li, Yao
Wilsterman, Eric J.
Shastry, Suresh
Dierksen, Gregory
Birket, Susan E.
Date Issued
January 2013
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Liu, Linbo et al. “Method for Quantitative Study of Airway Functional Microanatomy Using Micro-Optical Coherence Tomography.” Ed. Michael Myerburg. PLoS ONE 8.1 (2013): e54473.
Version
Final published version
Abstract
We demonstrate the use of a high resolution form of optical coherence tomography, termed micro-OCT (μOCT), for investigating the functional microanatomy of airway epithelia. μOCT captures several key parameters governing the function of the airway surface (airway surface liquid depth, periciliary liquid depth, ciliary function including beat frequency, and mucociliary transport rate) from the same series of images and without exogenous particles or labels, enabling non-invasive study of dynamic phenomena. Additionally, the high resolution of μOCT reveals distinguishable phases of the ciliary stroke pattern and glandular extrusion. Images and functional measurements from primary human bronchial epithelial cell cultures and excised tissue are presented and compared with measurements using existing gold standard methods. Active secretion from mucus glands in tissue, a key parameter of epithelial function, was also observed and quantified.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0054473
