Imaging cellular ultrastructures using expansion microscopy (U-ExM)
Name
emss-80371.pdf
Description
Accepted version
Size
1.76 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Gambarotto, Davide
Zwettler, Fabian U.
Le Guennec, Maeva
Schmidt-Cernohorska, Marketa
Fortun, Denis
Heine, Jörn
Schloetel, Jan-Gero
Reuss, Matthias
Unser, Michael
Boyden, E
Date Issued
December 2018
Journal
Nature Methods
Publisher
Springer Nature
Citation
Gambarotto, Davide et al. "Imaging cellular ultrastructures using expansion microscopy (U-ExM)." Nature Methods 16, 1 (December 2018): 71-74 © 2018 The Author(s)
Version
Author's final manuscript
Abstract
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41592-018-0238-1
