Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging
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boe-9-4-1492.pdf
Description
Published version
Size
4.94 MB
Format
Adobe PDF
Checksum (MD5)
f1fa1cf60272bb83d68ed0750044ab1d
Author(s)
Ohayon, Shay
DiCarlo, James
Date Issued
April 2018
Journal
Biomedical optics express
Publisher
The Optical Society
Citation
Ohayon, Shay et al. “Minimally invasive multimode optical fiber microendoscope for deep brain fluorescence imaging.” Biomedical optics express, vol. 9, no. 4, 2018, article 315080 © 2018 The Author(s)
Version
Final published version
Abstract
A major open challenge in neuroscience is the ability to measure and perturb neural activity in vivo from well defined neural sub-populations at cellular resolution anywhere in the brain. However, limitations posed by scattering and absorption prohibit non-invasive multi-photon approaches for deep (>2mm) structures, while gradient refractive index (GRIN) endoscopes are relatively thick and can cause significant damage upon insertion. Here, we present a novel micro-endoscope design to image neural activity at arbitrary depths via an ultra-thin multi-mode optical fiber (MMF) probe that has 5-10X thinner diameter than commercially available microendoscopes. We demonstrate micron-scale resolution, multi-spectral and volumetric imaging. In contrast to previous approaches, we show that this method has an improved acquisition speed that is sufficient to capture rapid neuronal dynamics in-vivo in rodents expressing a genetically encoded calcium indicator (GCaMP). Our results emphasize the potential of this technology in neuroscience applications and open up possibilities for cellular resolution imaging in previously unreachable brain regions.
MIT Department
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1364/BOE.9.001492
