Lipid bilayer and cytoskeletal interactions in a red blood cell
Name
Peng-2013-Lipid bilayer and cytoskeletal interactions in a red blood cell.pdf
Size
799.41 KB
Format
Adobe PDF
Checksum (MD5)
3d039fc35c4d36b6bcb221fb541000bc
Author(s)
Peng, Zhangli
Dao, Ming
Suresh, Subra
Li, Xuejin
Pivkin, Igor V.
Karniadakis, George E.
Date Issued
August 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Peng, Z., X. Li, I. V. Pivkin, M. Dao, G. E. Karniadakis, and S. Suresh. “Lipid Bilayer and Cytoskeletal Interactions in a Red Blood Cell.” Proceedings of the National Academy of Sciences 110, no. 33 (August 13, 2013): 13356–13361.
Version
Final published version
Abstract
We study the biomechanical interactions between the lipid bilayer and the cytoskeleton in a red blood cell (RBC) by developing a general framework for mesoscopic simulations. We treated the lipid bilayer and the cytoskeleton as two distinct components and developed a unique whole-cell model of the RBC, using dissipative particle dynamics (DPD). The model is validated by comparing the predicted results with measurements from four different and independent experiments. First, we simulated the micropipette aspiration and quantified the cytoskeletal deformation. Second, we studied the membrane fluctuations of healthy RBCs and RBCs parasitized to different intraerythrocytic stages by the malaria-inducing parasite Plasmodium falciparum. Third, we subjected the RBC to shear flow and investigated the dependence of its tank-treading frequency on shear rate. Finally, we simulated the bilayer–cytoskeletal detachment in channel flow to quantify the strength of such interactions when the corresponding bonds break. Taken together, these experiments and corresponding systematic DPD simulations probe the governing constitutive response of the cytoskeleton, elastic stiffness, viscous friction, and strength of bilayer–cytoskeletal interactions as well as membrane viscosities. Hence, the DPD simulations and comparisons with available independent experiments serve as validation of the unique two-component model and lead to useful insights into the biomechanical interactions between the lipid bilayer and the cytoskeleton of the RBC. Furthermore, they provide a basis for further studies to probe cell mechanistic processes in health and disease in a manner that guides the design and interpretation of experiments and to develop simulations of phenomena that cannot be studied systematically by experiments alone.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.1311827110
