Activation of a signaling pathway by the physical translocation of a chromosome
Name
nihms-1724009.pdf
Description
Accepted version
Size
1.37 MB
Format
Adobe PDF
Checksum (MD5)
0a5b00d61bf3228ef155e200d5fd5fec
Author(s)
Guzzo, Mathilde
Sanderlin, Allen G
Castro, Lennice K
Laub, Michael T
Date Issued
2021
Journal
Developmental Cell
Publisher
Elsevier BV
Citation
Guzzo, Mathilde, Sanderlin, Allen G, Castro, Lennice K and Laub, Michael T. 2021. "Activation of a signaling pathway by the physical translocation of a chromosome." Developmental Cell, 56 (15).
Version
Author's final manuscript
Abstract
In every organism, the cell cycle requires the execution of multiple processes in a strictly defined order. However, the mechanisms used to ensure such order remain poorly understood, particularly in bacteria. Here, we show that the activation of the essential CtrA signaling pathway that triggers cell division in Caulobacter crescentus is intrinsically coupled to the initiation of DNA replication via the physical translocation of a newly replicated chromosome, powered by the ParABS system. We demonstrate that ParA accumulation at the new cell pole during chromosome segregation recruits ChpT, an intermediate component of the CtrA signaling pathway. ChpT is normally restricted from accessing the selective PopZ polar microdomain until the new chromosome and ParA arrive. Consequently, any disruption to DNA replication initiation prevents ChpT polarization and, in turn, cell division. Collectively, our findings reveal how major cell-cycle events are coordinated in Caulobacter and, importantly, how chromosome translocation triggers an essential signaling pathway.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Howard Hughes Medical Institute
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.DEVCEL.2021.06.014
