Microfluidic bioprinting of tough hydrogel-based vascular conduits for functional blood vessels
Name
sciadv.abq6900.pdf
Description
Published version
Size
3.93 MB
Format
Adobe PDF
Checksum (MD5)
80ca59fa215dc5cecc745eb8153165c6
Author(s)
Wang, Di
Maharjan, Sushila
Kuang, Xiao
Wang, Zixuan
Mille, Luis S
Tao, Ming
Yu, Peng
Cao, Xia
Lian, Liming
Lv, Li
Date Issued
October 28, 2022
Journal
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Wang, Di, Maharjan, Sushila, Kuang, Xiao, Wang, Zixuan, Mille, Luis S et al. 2022. "Microfluidic bioprinting of tough hydrogel-based vascular conduits for functional blood vessels." Science Advances, 8 (43).
Version
Final published version
Abstract
Three-dimensional (3D) bioprinting of vascular tissues that are mechanically and functionally comparable to their native counterparts is an unmet challenge. Here, we developed a tough double-network hydrogel (bio)ink for microfluidic (bio)printing of mono- and dual-layered hollow conduits to recreate vein- and artery-like tissues, respectively. The tough hydrogel consisted of energy-dissipative ionically cross-linked alginate and elastic enzyme–cross-linked gelatin. The 3D bioprinted venous and arterial conduits exhibited key functionalities of respective vessels including relevant mechanical properties, perfusability, barrier performance, expressions of specific markers, and susceptibility to severe acute respiratory syndrome coronavirus 2 pseudo-viral infection. Notably, the arterial conduits revealed physiological vasoconstriction and vasodilatation responses. We further explored the feasibility of these conduits for vascular anastomosis. Together, our study presents biofabrication of mechanically and functionally relevant vascular conduits, showcasing their potentials as vascular models for disease studies in vitro and as grafts for vascular surgeries in vivo, possibly serving broad biomedical applications in the future.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1126/sciadv.abq6900
