One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
Name
jem_20172064.pdf
Description
Published version
Size
2.33 MB
Format
Adobe PDF
Checksum (MD5)
6b05d1bd768feb8adb33452117f8ad98
Author(s)
Jacobsen, Johanne T.
Mesin, Luka
Markoulaki, Styliani
Schiepers, Ariën
Cavazzoni, Cecília B.
Bousbaine, Djenet
Jaenisch, Rudolf
Victora, Gabriel D.
Date Issued
September 2018
Journal
Journal of Experimental Medicine
Publisher
Rockefeller University Press
Citation
Jacobsen, Johanne T. et al. "One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation." Journal of Experimental Medicine 215, 10 (September 2018): 2686–2695 © 2018 Jacobsen et al
Version
Final published version
Abstract
We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes with the ability to study Ig SHM, CSR, and affinity maturation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1084/jem.20172064
