Zebrafish promoter microarrays identify actively transcribed embryonic genes
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gb-2006-7-8-r71.pdf
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Author(s)
Wardle, Fiona C
Odom, Duncan
Bell, George W.
Wiellette, Elizabeth L
Herbolsheimer, Elizabeth
Smith, James C
Yuan, Bingbing B.
Sive, Hazel L.
Danford, Timothy William
Young, Richard A.
Date Issued
August 2006
Journal
Genome Biology
Publisher
BioMed Central Ltd
Citation
Genome Biology. 2006 Aug 04;7(8):R71
Version
Final published version
Abstract
We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/gb-2006-7-8-r71
