CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
Name
document.pdf
Description
Published version
Size
1.93 MB
Format
Adobe PDF
Checksum (MD5)
57cf9e47deb56b45e0ff192e45e1ac9f
Author(s)
Yin, Hao
Wu, Qiongqiong
Anderson, Daniel Griffith
Weng, Zhiping
Date Issued
June 14, 2017
Journal
Genome Biology
Publisher
Springer Nature
Citation
Mou, Haiwei, Jordan L. Smith, Lingtao Peng, Hao Yin, Jill Moore, Xiao-Ou Zhang, Chun-Qing Song,Ankur Sheel, Qiongqiong Wu, Deniz M. Ozata, Yingxiang Li, Daniel G. Anderson, Charles P. Emerson, Erik J. Sontheimer, Melissa J. Moore,*, Zhiping Weng and Wen Xue. "CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion." Genome Biology, 18, no. 1 (June 2017): article no. 108 © 2017 The Author(s)
Version
Final published version
Abstract
CRISPR is widely used to disrupt gene function by inducing small insertions and deletions. Here, we show that some single-guide RNAs (sgRNAs) can induce exon skipping or large genomic deletions that delete exons. For example, CRISPR-mediated editing of β-catenin exon 3, which encodes an autoinhibitory domain, induces partial skipping of the in-frame exon and nuclear accumulation of β-catenin. A single sgRNA can induce small insertions or deletions that partially alter splicing or unexpected larger deletions that remove exons. Exon skipping adds to the unexpected outcomes that must be accounted for, and perhaps taken advantage of, in CRISPR experiments.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/s13059-017-1237-8
