Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions
Name
acscentsci.9b00059.pdf
Description
Published version
Size
3.95 MB
Format
Adobe PDF
Checksum (MD5)
46bda6bf70b55524a18e9b4deb9b11dd
Author(s)
Zeininger, Lukas
Nagelberg, Sara Nicole
Harvey, Kent S.
Savagatrup, Suchol
Herbert, Myles Benton
Yoshinaga, Kosuke
Kolle, Mathias
Swager, Timothy M
Date Issued
April 2019
Journal
ACS central science
Publisher
American Chemical Society (ACS)
Citation
Zeininger, Lukas et al. “Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions.” ACS central science 5 (2019): 789-795 © 2019 The Author(s)
Version
Final published version
Abstract
Reliable early-stage detection of foodborne pathogens is a global public health challenge that requires new and improved sensing strategies. Here, we demonstrate that dynamically reconfigurable fluorescent double emulsions can function as highly responsive optical sensors for the rapid detection of carbohydrates fructose, glucose, mannose, and mannan, which are involved in many biological and pathogenic phenomena. The proposed detection strategy relies on reversible reactions between boronic acid surfactants and carbohydrates at the hydrocarbon/water interface leading to a dynamic reconfiguration of the droplet morphology, which alters the angular distribution of the droplet's fluorescent light emission. We exploit this unique chemical-morphological-optical coupling to detect Salmonella enterica, a type of bacteria with a well-known binding affinity for mannose. We further demonstrate an oriented immobilization of antibodies at the droplet interface to permit higher selectivity. Our demonstrations yield a new, inexpensive, robust, and generalizable sensing strategy that can help to facilitate the early detection of foodborne pathogens.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/ACSCENTSCI.9B00059
