Glial Ca2+signaling links endocytosis to K+ buffering around neuronal somas to regulate excitability
Name
elife-44186-v2.pdf
Description
Published version
Size
5.34 MB
Format
Adobe PDF
Checksum (MD5)
c36edf2f4fb2a4643e42f7f4fec11bf7
Author(s)
Weiss Sharabi, Shirley
Melom, Jan Elizabeth
Ormerod, Kiel G
Zhang, Yao
Littleton, J. Troy
Date Issued
April 2019
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Weiss, Shirley et al. “Glial Ca2+signaling links endocytosis to K+ buffering around neuronal somas to regulate excitability.” eLife 8 (2019): e44186 © 2019 The Author(s)
Version
Final published version
Abstract
Glial-neuronal signaling at synapses is widely studied, but how glia interact with neuronal somas to regulate their activity is unclear. Drosophila cortex glia are restricted to brain regions devoid of synapses, providing an opportunity to characterize interactions with neuronal somas. Mutations in the cortex glial NCKXzydecoelevate basal Ca2+, predisposing animals to seizure-like behavior. To determine how cortex glial Ca2+signaling controls neuronal excitability, we performed an in vivo modifier screen of the NCKXzydecoseizure phenotype. We show that elevation of glial Ca2+causes hyperactivation of calcineurin-dependent endocytosis and accumulation of early endosomes. Knockdown of sandman, a K2P channel, recapitulates NCKXzydecoseizures. Indeed, sandman expression on cortex glial membranes is substantially reduced in NCKXzydecomutants, indicating enhanced internalization of sandman predisposes animals to seizures. These data provide an unexpected link between glial Ca2+signaling and the well-known role of glia in K+buffering as a key mechanism for regulating neuronal excitability.
MIT Department
Picower Institute for Learning and Memory
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.7554/ELIFE.44186
