Diversity, classification and evolution of CRISPR-Cas systems
Name
nihms883917.pdf
Description
Accepted version
Size
1.27 MB
Format
Adobe PDF
Checksum (MD5)
22aaa8a11abf76e68bb5bcfe0c36b5b0
Author(s)
Koonin, Eugene V
Makarova, Kira S
Zhang, Feng
Date Issued
June 2017
Journal
Current Opinion in Microbiology
Publisher
Elsevier BV
Citation
VKoonin, Eugene, Makarova, Kira S. and Zhang, Feng, "Diversity, classification and evolution of CRISPR-Cas systems." Current Opinion in Microbiology 37 (June 2017): 67-78 © 2017
Version
Author's final manuscript
Abstract
The bacterial and archaeal CRISPR-Cas systems of adaptive immunity show remarkable diversity of protein composition, effector complex structure, genome locus architecture and mechanisms of adaptation, pre-CRISPR (cr)RNA processing and interference. The CRISPR-Cas systems belong to two classes, with multi-subunit effector complexes in Class 1 and single-protein effector modules in Class 2. Concerted genomic and experimental efforts on comprehensive characterization of Class 2 CRISPR-Cas systems led to the identification of two new types and several subtypes. The newly characterized type VI systems are the first among the CRISPR-Cas variants to exclusively target RNA. Unexpectedly, in some of the class 2 systems, the effector protein is additionally responsible for the pre-crRNA processing. Comparative analysis of the effector complexes indicates that Class 2 systems evolved from mobile genetic elements on multiple, independent occasions.
MIT Department
Broad Institute of MIT and Harvard
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.mib.2017.05.008
