Antibody-Functionalized Fluid-Permeable Surfaces for Rolling Cell Capture at High Flow Rates
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Mittal-2012-Antibody-Functionali.pdf
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Author(s)
Mittal, Sukant
Wong, Ian Y.
Deen, William M.
Toner, Mehmet
Date Issued
February 2012
Journal
Biophysical Journal
Publisher
Elsevier
Citation
Mittal, Sukant, Ian Y. Wong, William M. Deen, and Mehmet Toner. “Antibody-Functionalized Fluid-Permeable Surfaces for Rolling Cell Capture at High Flow Rates.” Biophysical Journal 102, no. 4 (February 2012): 721–730. © 2012 Biophysical Society
Version
Final published version
Abstract
Adhesion-based cell capture on surfaces in microfluidic devices forms the basis of numerous biomedical diagnostics and in vitro assays. However, the performance of these platforms is partly limited by interfacial phenomena that occur at low Reynolds numbers. In contrast, cell homing to porous vasculature is highly effective in vivo during inflammation, stem cell trafficking, and cancer metastasis. Here, we show that a porous, fluid-permeable surface functionalized with cell-specific antibodies promotes efficient and selective cell capture in vitro. This architecture is advantageous due to enhanced transport as streamlines are diverted toward the surface. Moreover, specific cell-surface interactions are promoted due to reduced shear, allowing gentle cell rolling and arrest. Together, these synergistic effects enable highly effective cell capture at flow rates more than an order of magnitude larger than those provided by existing devices with solid surfaces.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.bpj.2011.12.044
