A genetically encoded near-infrared fluorescent calcium ion indicator
Name
nihms-1515854.pdf
Description
Accepted version
Size
1.52 MB
Format
Unknown
Checksum (MD5)
3d7bb7c3a2f631963b15ae58ae64a08e
Author(s)
Qian, Yong
Piatkevich, Kiryl D.
Mc Larney, Benedict
Abdelfattah, Ahmed S.
Mehta, Sohum
Murdock, Mitchell H.
Gottschalk, Sven
Molina, Rosana S.
Zhang, Wei
Chen, Yingche
Date Issued
January 2019
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Qian, Yong, Piatkevich, Kiryl D, Mc Larney, Benedict, Abdelfattah, Ahmed S, Mehta, Sohum et al. 2019. "A genetically encoded near-infrared fluorescent calcium ion indicator." Nature Methods, 16 (2).
Version
Author's final manuscript
Abstract
© 2019, The Author(s), under exclusive licence to Springer Nature America, Inc. We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41592-018-0294-6
