Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis
Name
elife-76664-v1.pdf
Description
Published version
Size
6.73 MB
Format
Adobe PDF
Checksum (MD5)
e472d7feb71282194715c1e7bf1e9a79
Author(s)
Miettinen, Teemu P
Ly, Kevin S
Lam, Alice
Manalis, Scott R
Date Issued
2022
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Miettinen, Teemu P, Ly, Kevin S, Lam, Alice and Manalis, Scott R. 2022. "Single-cell monitoring of dry mass and dry mass density reveals exocytosis of cellular dry contents in mitosis." eLife, 11.
Version
Final published version
Abstract
Cell mass and composition change with cell cycle progression. Our previous work characterized buoyant mass dynamics in mitosis (Miettinen et al., 2019), but how dry mass and cell composition change in mitosis has remained unclear. To better understand mitotic cell growth and compositional changes, we develop a single-cell approach for monitoring dry mass and the density of that dry mass every ~75 s with 1.3% and 0.3% measurement precision, respectively. We find that suspension grown mammalian cells lose dry mass and increase dry mass density following mitotic entry. These changes display large, non-genetic cell-to-cell variability, and the changes are reversed at metaphase-anaphase transition, after which dry mass continues accumulating. The change in dry mass density causes buoyant and dry mass to differ specifically in early mitosis, thus reconciling existing literature on mitotic cell growth. Mechanistically, cells in early mitosis increase lysosomal exocytosis, and inhibition of lysosomal exocytosis decreases the dry mass loss and dry mass density increase in mitosis. Overall, our work provides a new approach for monitoring single-cell dry mass and dry mass density, and reveals that mitosis is coupled to extensive exocytosis-mediated secretion of cellular contents.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.7554/ELIFE.76664
