Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration
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journal.pone.0011987 (1).PDF
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5.72 MB
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Adobe PDF
Checksum (MD5)
5f2210369d8bc3dd3a92ec11712ab2b8
Author(s)
Sun, Yutong
Lodish, Harvey F
Date Issued
August 2010
Journal
PLoS ONE
Publisher
Public Library of Science (PLoS)
Citation
Sun, Yutong, and Harvey F. Lodish. “Adiponectin Deficiency Promotes Tumor Growth in Mice by Reducing Macrophage Infiltration.” Edited by Samithamby Jeyaseelan. PLoS ONE 5, 8 (August 2010): e11987 © 2010 Sun, Lodish
Version
Final published version
Abstract
Adiponectin is an adipocyte-derived plasma protein that has been implicated in regulating angiogenesis, but the role of adiponectin in regulating this process is still controversial. In this study, in order to determine whether adiponectin affects tumor growth and tumor induced vascularization, we implanted B16F10 melanoma and Lewis Lung Carcinoma cells subcutaneously into adiponectin knockout and wild-type control mice, and found that adiponectin deficiency markedly promoted the growth of both tumors. Immunohistochemical analyses indicated that adiponectin deficiency reduced macrophage recruitment to the tumor, but did not affect cancer cell mitosis, apoptosis, or tumor-associated angiogenesis. In addition, treatment with recombinant adiponectin did not affect the proliferation of cultured B16F10 tumor cells. Importantly, the restoration of microphage infiltration at an early stage of tumorigenesis by means of co-injection of B16F10 cells and macrophages reversed the increased tumor growth in adiponectin knockout mice. Thus, we conclude that the enhanced tumor growth observed in adiponectin deficient mice is likely due to the reduction of macrophage infiltration rather than enhanced angiogenesis.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Attribution 4.0 International (CC BY 4.0)
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0011987
