Virtual k -Space Modulation Optical Microscopy
Name
PhysRevLett.117.028102.pdf
Size
2.22 MB
Format
Adobe PDF
Checksum (MD5)
4345ce5fbd78f71076a114b123f77e4f
Author(s)
Zheng, Guoan
Fang, Yue
Xu, Yingke
Liu, Xu
Kuang, Cuifang
Ma, Ye
Zhou, Renjie
So, Peter T. C.
Date Issued
July 2016
Journal
Physical Review Letters
Publisher
American Physical Society
Citation
Kuang, Cuifang et al. “Virtual K -Space Modulation Optical Microscopy.” Physical Review Letters 117.2 (2016): n. pag. © 2016 American Physical Society
Version
Final published version
Abstract
We report a novel superresolution microscopy approach for imaging fluorescence samples. The reported approach, termed virtual k-space modulation optical microscopy (VIKMOM), is able to improve the lateral resolution by a factor of 2, reduce the background level, improve the optical sectioning effect and correct for unknown optical aberrations. In the acquisition process of VIKMOM, we used a scanning confocal microscope setup with a 2D detector array to capture sample information at each scanned x-y position. In the recovery process of VIKMOM, we first modulated the captured data by virtual k-space coding and then employed a ptychography-inspired procedure to recover the sample information and correct for unknown optical aberrations. We demonstrated the performance of the reported approach by imaging fluorescent beads, fixed bovine pulmonary artery endothelial (BPAE) cells, and living human astrocytes (HA). As the VIKMOM approach is fully compatible with conventional confocal microscope setups, it may provide a turn-key solution for imaging biological samples with ∼100 nm lateral resolution, in two or three dimensions, with improved optical sectioning capabilities and aberration correcting.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
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DOI of Published Version
https://doi.org/10.1103/PhysRevLett.117.028102
