Differential translation of mRNA isoforms transcribed with distinct sigma factors
Name
RNA-2021-McCormick-791-804.pdf
Description
Published version
Size
2.14 MB
Format
Unknown
Checksum (MD5)
6ba1f16bd3cb9403826879eea68f4582
Author(s)
McCormick, Dylan M
Lalanne, Jean-Benoit
Lan, Tammy CT
Rouskin, Silvi
Li, Gene-Wei
Date Issued
2021
Journal
RNA
Publisher
Cold Spring Harbor Laboratory
Version
Final published version
Abstract
Sigma factors are an important class of bacterial transcription factors that lend specificity to RNA polymerases by binding to distinct promoter elements for genes in their regulons. Here we show that activation of the general stress sigma factor, σB, in Bacillus subtilis paradoxically leads to dramatic induction of translation for a subset of its regulon genes. These genes are translationally repressed when transcribed by the housekeeping sigma factor, σA, owing to extended RNA secondary structures as determined in vivo using DMS-MaPseq. Transcription from σB-dependent promoters ablates the secondary structures and activates translation, leading to dual induction. Translation efficiencies between σB- and σA-dependent RNA isoforms can vary by up to 100-fold, which in multiple cases exceeds the magnitude of transcriptional induction. These results highlight the role of long-range RNA folding in modulating translation and demonstrate that a transcription factor can regulate protein synthesis beyond its effects on transcript levels.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Physics
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution NonCommercial License 4.0
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DOI of Published Version
https://doi.org/10.1261/rna.078747.121
