Effect of Gold Nanorod Surface Chemistry on Cellular Response
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grabinski_ACS_Nano_2011.pdf
Description
Main article
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4.41 MB
Format
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Author(s)
Grabinski, Christin
Schaeublin, Nicole
Wijaya, Andy
D’Couto, Helen
Baxamusa, Salmaan H.
Hamad-Schifferli, Kimberly
Hussain, Saber M.
Date Issued
April 2011
Journal
ACS Nano
Publisher
American Chemical Society
Citation
Grabinski, Christin et al. “Effect of Gold Nanorod Surface Chemistry on Cellular Response.” ACS Nano 5.4 (2011): 2870–2879.
Version
Author's final manuscript
Abstract
Gold nanorods (GNRs) stabilized with cetyltrimethylammonium bromide (CTAB) and GNR functionalized via a ligand exchange method with either thiolated polyethylene glycol (PEG5000) or mercaptohexadecanoic acid (MHDA) were investigated for their stability in biological media and subsequent toxicological effects to HaCaT cells. GNR-PEG and GNR-MHDA exhibited minimal effects on cell proliferation, whereas GNR-CTAB reduced cell proliferation significantly due to the inherent toxicity of the cationic surfactant to cells. Cell uptake studies indicated relatively low uptake for GNR-PEG and high uptake for GNR-MHDA. Reverse transcriptase polymerase chain reaction (RT-PCR) revealed that GNR-PEG induced less significant and unique changes in the transcription levels of 84 genes related to stress and toxicity compared to GNR-MHDA. The results demonstrate that, although cell proliferation was not affected by both particles, there is a significant difference in gene expression in GNR-MHDA exposed cells, suggesting long-term implications for chronic exposure.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/nn103476x
