Semiconductor-based DNA sequencing of histone modification states
Name
Regev_Semiconductor-based DNA.pdf
Size
1.11 MB
Format
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Checksum (MD5)
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Author(s)
Cheng, Christine S.
Rai, Kunal
Garber, Manuel
Hollinger, Andrew
Robbins, Dana
Anderson, Scott
Macbeth, Alyssa
Tzou, Austin
Carneiro, Mauricio O.
Raychowdhury, Raktima
Date Issued
October 2013
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Cheng, Christine S. et al. “Semiconductor-Based DNA Sequencing of Histone Modification States.” Nature Communications 4 (2013): n. pag.
Version
Author's final manuscript
Abstract
The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/ncomms3672
