Point-of-Care Devices to Detect Zika and Other Emerging Viruses
Name
combined text and figures_.pdf
Description
Accepted version
Size
1.78 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
de Puig Guixe, Helena
Bosch, Irene
Collins, James J.
Gehrke, Lee
Date Issued
June 2020
Journal
Annual Review of Biomedical Engineering
Publisher
Annual Reviews
Citation
de Puig, Helena et al. "Point-of-Care Devices to Detect Zika and Other Emerging Viruses." Annual Review of Biomedical Engineering 22, 1 (June 2020): 371-386. © 2020 Annual Reviews
Version
Author's final manuscript
Abstract
Rapid diagnostic tests (point-of-care devices) are critical components of informed patient care and public health monitoring (surveillance applications). We propose that among the many rapid diagnostics platforms that have been tested or are in development, lateral flow immunoassays and synthetic biology-based diagnostics (including CRISPR-based diagnostics) represent the best overall options given their ease of use, scalability for manufacturing, sensitivity, and specificity. This review describes the identification of lateral flow immunoassay monoclonal antibody pairs that detect and distinguish between closely related pathogens and that are used in combination with functionalized multicolored nanoparticles and computational methods to deconvolute data. We also highlight the promise of synthetic biology-based diagnostic tests, which use synthetic genetic circuits that activate upon recognition of a pathogen-associated nucleic acid sequence, and discuss how the combined or parallel use of lateral flow immunoassays and synthetic biology tools may represent the future of scalable rapid diagnostics.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1146/annurev-bioeng-060418-052240
