Bioelectronic measurement and feedback control of molecules in living cells
Name
s41598-017-12655-2.pdf
Size
1.81 MB
Format
Adobe PDF
Checksum (MD5)
49d6d3f6ae9d7acc8a7349146e0bb007
Author(s)
Banerjee, Areen
Sarpeshkar, Rahul
Weaver, Isaac
Thorsen, Todd A.
Date Issued
October 2017
Journal
Scientific Reports
Publisher
Nature Publishing Group
Citation
Banerjee, Areen, Isaac Weaver, Todd Thorsen, and Rahul Sarpeshkar. “Bioelectronic Measurement and Feedback Control of Molecules in Living Cells.” Scientific Reports 7, 1 (October 2017): 12511 © 2017 The Author(s)
Version
Final published version
Abstract
We describe an electrochemical measurement technique that enables bioelectronic measurements of reporter proteins in living cells as an alternative to traditional optical fluorescence. Using electronically programmable microfluidics, the measurement is in turn used to control the concentration of an inducer input that regulates production of the protein from a genetic promoter. The resulting bioelectronic and microfluidic negative-feedback loop then serves to regulate the concentration of the protein in the cell. We show measurements wherein a user-programmable set-point precisely alters the protein concentration in the cell with feedback-loop parameters affecting the dynamics of the closed-loop response in a predictable fashion. Our work does not require expensive optical fluorescence measurement techniques that are prone to toxicity in chronic settings, sophisticated time-lapse microscopy, or bulky/expensive chemo-stat instrumentation for dynamic measurement and control of biomolecules in cells. Therefore, it may be useful in creating a: Cheap, portable, chronic, dynamic, and precise all-electronic alternative for measurement and control of molecules in living cells.
MIT Department
Lincoln Laboratory
Terms of Use
Attribution 4.0 International (CC BY 4.0)
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/S41598-017-12655-2
