Synthetic scaffolds and protein assemblies for engineering applications
Name
59668886-MIT.pdf
Description
Full printable version
Size
4.98 MB
Format
Adobe PDF
Checksum (MD5)
705117708e193498db27d7cae2c28231
Author(s)
Norville, Julie Erin, 1980-
Advisor(s)
Thomas F. Knight, Jr. and Angela M. Belcher.
Date Issued
2004
Publisher
Massachusetts Institute of Technology
Abstract
S-layer proteins, which naturally self-assemble on the exterior of cells, provide an interesting basis for the creation of synthetic scaffolds. In this thesis, I created a plasmid which produces a recombinant form of a well characterized S layer protein, sbpA, which has a number of properties ideal for nanotechnology applications. I also explored purification of both the native and recombinant forms of sbpA. Together these preliminary studies are the first, necessary, steps towards quantitative generation of crystallization conditions and the ultimate modifications of the protein form for a wide variety of engineering applications.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2004.
Includes bibliographical references (p. 57-63).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
Persistent DSpace Link
