Characterization and optimization of PDMS microfluidic devices for rapid DNA hybridization
Name
62783474-MIT.pdf
Description
Full printable version
Size
3.64 MB
Format
Adobe PDF
Checksum (MD5)
6dfb7acbddc901429db3c3879d9e1872
Author(s)
Hu, Jenny (Jenny Ezu)
Advisor(s)
Todd Thorsen.
Date Issued
2005
Publisher
Massachusetts Institute of Technology
Abstract
Two elastomeric microfluidic devices were designed for the purpose of conducting rapid, flow-based, multiplexed DNA hybridization. Experimental results showed that flowing hybridization assays could detect similar concentrations of labeled probe as standard stationary microarrays, but in 1/100h of the time, using 2% of the sample volume. An 8-channel device was used to spot glass slides with 64 hybridization assays and generate data supporting a theoretical model of DNA hybridization in both traditional stationary microarrays and flowing sample arrays. Larger devices were also used to create rrays of 96x96 spots on a single slide, demonstrating the scalability of the technology. Protocols were written and optimized for the use of both chips, allowing the technology to be distributed to collaborating labs for further development.
Description
Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2005.
Includes bibliographical references (p. 51-53).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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