Fast gene transfer into the adult zebrafish brain by herpes simplex virus 1 (HSV-1) and electroporation: methods and optogenetic applications
Name
Zou-2014-Fast gene transfer.pdf
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2.29 MB
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Author(s)
Zou, Ming
De Koninck, Paul
Neve, Rachael L.
Friedrich, Rainer W.
Date Issued
May 2014
Journal
Frontiers in Neural Circuits
Publisher
Frontiers Research Foundation
Citation
Zou, Ming, Paul De Koninck, Rachael L. Neve, and Rainer W. Friedrich. “Fast Gene Transfer into the Adult Zebrafish Brain by Herpes Simplex Virus 1 (HSV-1) and Electroporation: Methods and Optogenetic Applications.” Front. Neural Circuits 8 (May 6, 2014).
Version
Final published version
Abstract
The zebrafish has various advantages as a model organism to analyze the structure and function of neural circuits but efficient viruses or other tools for fast gene transfer are lacking. We show that transgenes can be introduced directly into the adult zebrafish brain by herpes simplex type I viruses (HSV-1) or electroporation. We developed a new procedure to target electroporation to defined brain areas and identified promoters that produced strong long-term expression. The fast workflow of electroporation was exploited to express multiple channelrhodopsin-2 variants and genetically encoded calcium indicators in telencephalic neurons for measurements of neuronal activity and synaptic connectivity. The results demonstrate that HSV-1 and targeted electroporation are efficient tools for gene delivery into the zebrafish brain, similar to adeno-associated viruses and lentiviruses in other species. These methods fill an important gap in the spectrum of molecular tools for zebrafish and are likely to have a wide range of applications.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.3389/fncir.2014.00041
