Extending imaging depth of multiphoton microscopy
Name
232359016-MIT.pdf
Description
Full printable version
Size
8.28 MB
Format
Adobe PDF
Checksum (MD5)
9b17ff6dd7c019654784a48bbeae1043
Author(s)
Cha, Jae Won
Advisor(s)
Peter T.C. So.
Date Issued
2007
Publisher
Massachusetts Institute of Technology
Abstract
Two-photon excitation fluorescence microscopy has capability of deep tissue imaging with biological samples. However, because of the inhomogeneity of the refractive index in biological samples, the wavefront of the excitation light is often distorted. Due to the distortion of the wavefront, the point spread function at the focal point becomes broadened resulting in degraded resolution and lower signal. With an adaptive optics system, which consists of a wavefront camera and deformable mirror, the wavefront distortion can be measured and corrected. By correcting the distorted wavefront with adaptive optics, resolution and signal level can be preserved at greater imaging depth.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.
Includes bibliographical references (leaves 45-46).
Subjects
Mechanical Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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