DNA as a programmable material : de novo gene synthesis and error correction
Name
289478664-MIT.pdf
Description
Full printable version
Size
13.45 MB
Format
Adobe PDF
Checksum (MD5)
e3d50f095e5c8e984cc0617e05558ece
Author(s)
Hwang, Samuel James
Advisor(s)
Joseph Jacobson.
Alternative Title
Deoxyribonucleic acid as a programmable material
Date Issued
2008
Publisher
Massachusetts Institute of Technology
Abstract
Deoxyribonucleic acid (DNA), the polymeric molecule that carries the genetic code of all living organisms, is arguably one of the most programmable assembly materials available to chemists, biologists, and materials scientists. Scientists have used DNA to build many different structures for various applications in disparate areas of research from traditional biological applications to more recent non-biological applications. Although DNA isn't typically thought of as an assembly material by people not doing research in the area, the availability of decreasing cost synthetic oligonucleotides has led to advances in gene fabrication technology which in turn has enabled synthetic biology to flourish. Using DNA as a building material for small and large constructs of DNA is reliant on having effective gene synthesis techniques. Construction of synthetic DNA is limited by errors that pervade the final product. To address this problem, effective error correction methods are pivotal. Having extremely robust gene synthesis and error correction techniques will allow researchers to generate very large scale constructs potentially necessary in applications such as genome re-engineering.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2008.
Includes bibliographical references (leaves 42-43).
Subjects
Materials Science and Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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