Electrically addressable vesicles: Tools for dielectrophoresis metrology
Name
Voldman_Electrically addressable.pdf
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1.44 MB
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Adobe PDF
Checksum (MD5)
2304920bedf12f28b59acb4b79d54dc3
Author(s)
Desai, Salil P.
Vahey, Michael D.
Voldman, Joel
Date Issued
February 2009
Journal
Langmuir
Publisher
American Chemical Society (ACS)
Citation
Desai, Salil P., Michael D. Vahey, and Joel Voldman. “Electrically Addressable Vesicles: Tools for Dielectrophoresis Metrology.” Langmuir 25.6 (2009): 3867–3875.
Version
Author's final manuscript
Abstract
Dielectrophoresis (DEP) has emerged as an important tool for the manipulation of bioparticles ranging from the submicron to the tens of microns in size. Here we show the use of phospholipid vesicle electroformation techniques to develop a new class of test particles with specifically engineered electrical propserties to enable identifiable dielectrophoretic responses in microfabricated systems. These electrically addressable vesicles (EAVs) enable the creation of electrically distinct populations of test particles for DEP. EAVs offer control of both their inner aqueous core and outer membrane properties; by encapsulating solutions of different electrolyte strength inside the vesicle and by incorporating functionalized phospholipids containing poly(ethylene glycol) (PEG) brushes attached to their hydrophilic headgroup in the vesicle membrane, we demonstrate control of the vesicles’ electrical polarizabilities. This combined with the ability to encode information about the properties of the vesicle in its fluorescence signature forms the first steps toward the development of EAV populations as metrology tools for any DEP-based microsystem.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1021/la803330c
