New pulsed EPR methods and their application to characterize mitochondrial complex I
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Maly-2009-New pulsed EPR metho.pdf
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Author(s)
Maly, Thorsten
Zwicker, Klaus
Cernescu, Adrian
Brandt, Ulrich
Prisner, Thomas F.
Date Issued
February 2009
Journal
Biochimica et Biophysica Acta (BBA) - Bioenergetics
Publisher
Elsevier
Citation
Maly, Thorsten, Klaus Zwicker, Adrian Cernescu, Ulrich Brandt, and Thomas F. Prisner. “New Pulsed EPR Methods and Their Application to Characterize Mitochondrial Complex I.” Biochimica et Biophysica Acta (BBA) - Bioenergetics 1787, no. 6 (June 2009): 584–592. © 2009 Elsevier B.V.
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Final published version
Abstract
Electron Paramagnetic Resonance (EPR) spectroscopy is the method of choice to study paramagnetic cofactors that often play an important role as active centers in electron transfer processes in biological systems. However, in many cases more than one paramagnetic species is contributing to the observed EPR spectrum, making the analysis of individual contributions difficult and in some cases impossible. With time-domain techniques it is possible to exploit differences in the relaxation behavior of different paramagnetic species to distinguish between them and separate their individual spectral contribution. Here we give an overview of the use of pulsed EPR spectroscopy to study the iron–sulfur clusters of NADH:ubiquinone oxidoreductase (complex I). While FeS cluster N1 can be studied individually at a temperature of 30 K, this is not possible for FeS cluster N2 due to its severe spectral overlap with cluster N1. In this case Relaxation Filtered Hyperfine (REFINE) spectroscopy can be used to separate the overlapping spectra based on differences in their relaxation behavior.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Francis Bitter Magnet Laboratory (Massachusetts Institute of Technology)
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.bbabio.2009.02.003
