Helical nanofiber yarn enabling highly stretchable engineered microtissue
Name
9245.full.pdf
Description
Published version
Size
1.51 MB
Format
Adobe PDF
Checksum (MD5)
c4a838b4a9a8f7e4ff3be411c41f5c96
Author(s)
Li, Yiwei
Hao, Yukun
Gupta, Satish Kumar
Hu, Jiliang
Wang, Yaqiong
Guo, Ming
Date Issued
April 24, 2019
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
Proceedings of the National Academy of Sciences
Citation
Li, Yiwei et al. "Helical nanofiber yarn enabling highly stretchable engineered microtissue." Proceedings of the National Academy of Sciences of the United States of America 116 (2019):9245-9250 © 2019 The Author(s)
Version
Final published version
Abstract
Development of microtissues that possess mechanical properties mimicking those of native stretchable tissues, such as muscle and tendon, is in high demand for tissue engineering and regenerative medicine. However, regardless of the significant advances in synthetic biomaterials, it remains challenging to fabricate living microtissue with high stretchability because application of large strains to microtissues can damage the cells by rupturing their structures. Inspired by the hierarchical helical structure of native fibrous tissues and its behavior of nonaffine deformation, we develop a highly stretchable and tough microtissue fiber made up of a hierarchical helix yarn scaffold, scaling from nanometers to millimeters, that can overcome this limitation. This microtissue can be stretched up to 15 times its initial length and has a toughness of 57 GJ m−3. More importantly, cells grown on this scaffold maintain high viability, even under severe cyclic strains (up to 600%) that can be attributed to the nonaffine deformation under large strains, mimicking native biopolymer scaffolds. Furthermore, as proof of principle, we demonstrate that the nanotopography of the helical nanofiber yarn is able to induce cytoskeletal alignment and nuclear elongation, which promote myogenic differentiation of mesenchymal stem cells by triggering nuclear translocation of transcriptional coactivator with PDZ-binding motif (TAZ). The highly stretchable microtissues we develop here will facilitate a variety of tissue engineering applications and the development of engineered living systems.
Subjects
Multidisciplinary
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.1821617116
