Engineering of a high lipid producing Yarrowia lipolytica strain
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document(1).pdf
Description
Published version
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2.45 MB
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Checksum (MD5)
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Author(s)
Friedlander, Jonathan
Tsakraklides, Vasiliki
Kamineni, Annapurna
Greenhagen, Emily H.
Consiglio, Andrew L.
MacEwen, Kyle
Crabtree, Donald V.
Afshar, Jonathan
Nugent, Rebecca L.
Hamilton, Maureen A.
Date Issued
March 2016
Journal
Biotechnology for Biofuels
Publisher
Springer Nature
Citation
Friedlander, Jonathan, Vasiliki Tsakraklides, Annapurna Kamineni et al. "Engineering of a high lipid producing Yarrowia lipolytica strain" Biotechnology for Biofuels, 9,77 (March 2016): p. 1-12. © 2016 Friedlander et al.
Version
Final published version
Abstract
Background: Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. Results: We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overexpression that applied combinatorial screening of overexpression and deletion genetic targets to construct a high lipid producing yeast biocatalyst. The resulting strain, NS432, combines overexpression of a heterologous DGA1 enzyme from Rhodosporidium toruloides, a heterlogous DGA2 enzyme from Claviceps purpurea, and deletion of the native TGL3 lipase regulator. These three genetic modifications, selected for their effect on lipid production, enabled a 77 % lipid content and 0.21 g lipid per g glucose yield in batch fermentation. In fed-batch glucose fermentation NS432 produced 85 g/L lipid at a productivity of 0.73 g/L/h. Conclusions: The yields, productivities, and titers reported in this study may further support the applied goal of cost effective, large -scale microbial lipid production for use as biofuels and biochemicals. Keywords: Yarrowia lipolytica, Lipid accumulation, Oleaginous yeast, Metabolic engineering
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1186/S13068-016-0492-3
