Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A translation
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nihms-1546343.pdf
Description
Accepted version
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3.21 MB
Format
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Author(s)
Basak, Anindita
Munschauer, Mathias
Lareau, Caleb A.
Montbleau, Kara E.
Ulirsch, Jacob C.
Hartigan, Christina R.
Schenone, Monica
Lian, John
Wang, Yaomei
Huang, Yumin
Date Issued
January 2020
Journal
Nature Genetics
Publisher
Springer Science and Business Media LLC
Citation
Basak, Anindita et al. "Control of human hemoglobin switching by LIN28B-mediated regulation of BCL11A translation." Nature Genetics 52, 2 (January 2020): 138–145 © 2020 The Author(s)
Version
Author's final manuscript
Abstract
Increased production of fetal hemoglobin (HbF) can ameliorate the severity of sickle cell disease and β-thalassemia1. BCL11A represses the genes encoding HbF and regulates human hemoglobin switching through variation in its expression during development2–7. However, the mechanisms underlying the developmental expression of BCL11A remain mysterious. Here we show that BCL11A is regulated at the level of messenger RNA (mRNA) translation during human hematopoietic development. Despite decreased BCL11A protein synthesis earlier in development, BCL11A mRNA continues to be associated with ribosomes. Through unbiased genomic and proteomic analyses, we demonstrate that the RNA-binding protein LIN28B, which is developmentally expressed in a pattern reciprocal to that of BCL11A, directly interacts with ribosomes and BCL11A mRNA. Furthermore, we show that BCL11A mRNA translation is suppressed by LIN28B through direct interactions, independently of its role in regulating let-7 microRNAs, and that BCL11A is the major target of LIN28B-mediated HbF induction. Our results reveal a previously unappreciated mechanism underlying human hemoglobin switching that illuminates new therapeutic opportunities.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
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DOI of Published Version
https://doi.org/10.1038/s41588-019-0568-7
