Epigenetic and post-transcriptional regulation of gene expression in pluripotent stem cells, differentiation and metastasis
Name
892972629-MIT.pdf
Description
Full printable version
Size
28.84 MB
Format
Adobe PDF
Checksum (MD5)
6e879ef43eef7a86517e535d7347ef69
Author(s)
Cheng, Wu Albert
Advisor(s)
Rudolf Jaenisch.
Date Issued
2014
Publisher
Massachusetts Institute of Technology
Abstract
Transmission of information from DNA to RNA to protein underlies the core of modem life forms. The advance in sequencing and genetic technologies has revolutionized the study of molecular biology, genetics and developmental biology enabling delineation of biological processes in unprecedented details. Through the study of epigenetics and posttranscriptional regulation of gene expression by high-throughput sequencing technologies in several biological processes, namely embryonic stem cells, somatic reprogramming, erythroid differentiation, epithelial-mesenchymal transition and cancer metastasis, this thesis work has identified novel players and regulatory mechanisms underlying these developmental processes and diseases. Furthermore, an attempt to engineer CRISPRzymes - protein fusions of RNA-guided DNA binding dCas9 - will enable experiments to directly test biological processes at defined genomic loci and expands the toolbox for synthetic biology and potentially opens up opportunities for novel therapeutics.
Description
Thesis: Ph. D., Massachusetts Institute of Technology, Computational and Systems Biology Program, 2014.
Cataloged from PDF version of thesis.
Includes bibliographical references.
Subjects
Computational and Systems Biology Program.
MIT Department
Massachusetts Institute of Technology. Computational and Systems Biology Program
Terms of Use
M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission.
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