Insights into GATA-1 Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis
Name
Yu et al Molecular Cell.pdf
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Author(s)
Yu, Ming
Riva, Laura
Schindler, Yocheved
Moran, Tyler B.
Cheng, Yong
Yu, Duonan
Hardison, Ross C.
Weiss, Mitchell J.
Orkin, Stuart H.
Bernstein, Bradley E.
Date Issued
November 2009
Journal
Molecular Cell
Publisher
Elsevier
Citation
Yu, Ming et al. “Insights into GATA-1-Mediated Gene Activation versus Repression via Genome-wide Chromatin Occupancy Analysis.” Molecular Cell 36.4 (2009): 682-695.
Version
Author's final manuscript
Abstract
The transcription factor GATA-1 is required for terminal erythroid maturation and functions
as an activator or repressor depending on gene context. Yet its in vivo site selectivity and ability
to distinguish between activated versus repressed genes remain incompletely understood. In this
study, we performed GATA-1 ChIP-seq in erythroid cells and compared it to GATA-1 induced
gene expression changes. Bound and differentially expressed genes contain a greater number of
GATA binding motifs, a higher frequency of palindromic GATA sites, and closer occupancy to
the transcriptional start site versus non-differentially expressed genes. Moreover, we show that
the transcription factor Zbtb7a occupies GATA-1 bound regions of some direct GATA-1 target
genes, that the presence of SCL/TAL1 helps distinguish transcriptional activation versus
repression, and that Polycomb Repressive Complex 2 (PRC2) is involved in epigenetic silencing
of a subset of GATA-1 repressed genes. These data provide insights into GATA-1 mediated
gene regulation in vivo.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1016/j.molcel.2009.11.002
