tRNAs Marked with CCACCA Are Targeted for Degradation
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Sharp_tRNAs marked.pdf
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Author(s)
Whipple, J. M.
Phizicky, E. M.
Wilusz, Jeremy
Sharp, Phillip A.
Date Issued
November 2011
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Wilusz, J. E., J. M. Whipple, E. M. Phizicky, and P. A. Sharp. “tRNAs Marked with CCACCA Are Targeted for Degradation.” Science 334, no. 6057 (November 10, 2011): 817-821.
Version
Author's final manuscript
Abstract
The CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase] adds CCA to the 3′ ends of transfer RNAs (tRNAs), a critical step in tRNA biogenesis that generates the amino acid attachment site. We found that the CCA-adding enzyme plays a key role in tRNA quality control by selectively marking structurally unstable tRNAs and tRNA-like small RNAs for degradation. Instead of adding CCA to the 3′ ends of these transcripts, CCA-adding enzymes from all three kingdoms of life add CCACCA. In addition, hypomodified mature tRNAs are subjected to CCACCA addition as part of a rapid tRNA decay pathway in vivo. We conjecture that CCACCA addition is a universal mechanism for controlling tRNA levels and preventing errors in translation.
Description
Author Manuscript 2012 May 11
MIT Department
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1213671
