Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array
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nihms-827969.pdf
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794.57 KB
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Author(s)
Mohanraju, Prarthana
Kneppers, Jeroen
DeGennaro, Ellen M
Wu, Wen Y
Scott, David A
Severinov, Konstantin
van der Oost, John
Zetsche, Bernd
Heidenreich, Matthias
Fedorova, Iana
Date Issued
December 2016
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Zetsche, Bernd et al. “Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA Array.” Nature Biotechnology 35, 1 (December 2016): 31–34 © 2017 Nature America, Inc, part of Springer Nature
Version
Author's final manuscript
Abstract
Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/NBT.3737
