Rickettsia parkeri forms extensive, stable contacts with the rough endoplasmic reticulum
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jcb_202406122 (1).pdf
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Published version
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5.18 MB
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Author(s)
Acevedo-Sánchez, Yamilex
Woida, Patrick J
Anderson, Caroline
Kraemer, Stephan
Lamason, Rebecca L
Date Issued
March 3, 2025
Journal
Journal of Cell Biology
Publisher
Rockefeller University Press
Citation
Yamilex Acevedo-Sánchez, Patrick J. Woida, Caroline Anderson, Stephan Kraemer, Rebecca L. Lamason; Rickettsia parkeri forms extensive, stable contacts with the rough endoplasmic reticulum. J Cell Biol 3 March 2025; 224 (3): e202406122.
Version
Final published version
Abstract
Upon invasion into the host cell, a subset of bacterial pathogens resides exclusively in the cytosol. While previous research revealed how they reshape the plasma membrane during invasion, subvert the immune response, and hijack cytoskeletal dynamics to promote their motility, it was unclear if these pathogens also interacted with the organelles in this crowded intracellular space. Here, we examined if the obligate intracellular pathogen Rickettsia parkeri interacts with the endoplasmic reticulum (ER), a large and dynamic organelle spread throughout the cell. Using live-cell microscopy and transmission and focused-ion-beam scanning electron microscopy, we show that R. parkeri forms extensive contacts with the rough ER that are ∼55 nm apart and cover more than half the bacterial surface. Depletion of the ER-specific tethers VAPA and VAPB reduced rickettsia–ER contacts, and VAPA and VAPB were localized around intracellular rickettsiae. Overall, our findings illuminate an interkingdom ER contact uniquely mediated by rickettsiae that mimics some characteristics of traditional host membrane contact sites.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Microbiology Graduate Program
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DOI of Published Version
https://doi.org/10.1083/jcb.202406122
