Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc
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Author(s)
Radford, Robert J.
Lippard, Stephen J.
Loas, Andrei Ioan
Date Issued
June 2014
Journal
Inorganic Chemistry
Publisher
American Chemical Society (ACS)
Citation
Loas, Andrei, Robert J. Radford, and Stephen J. Lippard. “Addition of a Second Binding Site Increases the Dynamic Range but Alters the Cellular Localization of a Red Fluorescent Probe for Mobile Zinc.” Inorg. Chem. 53, no. 13 (July 7, 2014): 6491–6493. © 2014 American Chemical Society
Version
Final published version
Abstract
We report the synthesis and photophysical properties of ZBR4 and ZR1, two resorufin-based ditopic probes for mobile zinc. Upon binding Zn[superscript 2+], the sensors display 14- and 41-fold enhancements of their red fluorescence emission, respectively. In contrast to ZR1 and other members of the ZBR family, which accumulate in the endoplasmic reticulum, ZBR4 spontaneously localizes to the mitochondria of HeLa cells. The modular approach in designing the constructs facilitates a homologation strategy aimed at tuning the zinc-binding and intracellular targeting properties of future probes.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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DOI of Published Version
https://doi.org/10.1021/ic500732z
