A New Toolbox for Assessing Single Cells
Name
nihms657494.pdf
Description
Accepted version
Size
1.95 MB
Format
Adobe PDF
Checksum (MD5)
54de2f71605970d54843ed19e555ca4b
Author(s)
Tsioris, Konstantinos
Torres, Alexis J
Douce, Thomas B
Love, J Christopher
Date Issued
2014
Journal
The Annual Review of Chemical and Biomolecular Engineering
Publisher
Annual Reviews
Citation
Tsioris, Konstantinos, et al. "A New Toolbox for Assessing Single Cells." Annual Review of Chemical and Biomolecular Engineering 5 (2014): 455-77.
Version
Author's final manuscript
Abstract
Unprecedented access to the biology of single cells is now feasible, enabled by recent technological advancements that allow us to manipulate and measure sparse samples and achieve a new level of resolution in space and time. This review focuses on advances in tools to study single cells for specific areas of biology. We examine both mature and nascent techniques to study single cells at the genomics, transcriptomics, and proteomics level. In addition, we provide an overview of tools that are well suited for following biological responses to defined perturbations with single-cell resolution. Techniques to analyze and manipulate single cells through soluble and chemical ligands, the microenvironment, and cell-cell interactions are provided. For each of these topics, we highlight the biological motivation, applications, methods, recent advances, and opportunities for improvement. The toolbox presented in this review can function as a starting point for the design of single-cell experiments. © 2014 by Annual Reviews.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Ragon Institute of MGH, MIT and Harvard
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1146/ANNUREV-CHEMBIOENG-060713-035958
