High-throughput multiplexed transcriptome and protein measurements in single cells
Name
1078636279-MIT.pdf
Description
Full printable version
Size
3.88 MB
Format
Adobe PDF
Checksum (MD5)
3a304ae5658ec8b63dfc3c8ac0577096
Author(s)
Shivaei, Shirin
Advisor(s)
Fei Chen.
Date Issued
2018
Publisher
Massachusetts Institute of Technology
Abstract
We present cell gels, a platform for high-throughput multiplexed measurements of transcriptome and proteome in single cells. This method takes advantage of droplet barcoding and hydrogel chemistry to capture mRNAs and proteins in thousands of cells. We report the challenges of acquiring sequencing data from cell gels due to complex mechanisms underlying bead based library prep in polyacrylamide gels. We show the applications of this method in detecting intracellular proteins, sorting based on intracellular markers after cell lysis, and expanding thousands of cells in single droplets.
Description
Thesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2018.
This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.
Cataloged from student-submitted PDF version of thesis.
Includes bibliographical references (pages 45-47).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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