High-definition spatial transcriptomics for in situ tissue profiling
Name
nihms-1536533.pdf
Description
Accepted version
Size
1002.36 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Vickovic, Sanja
Eraslan, Gökcen
Salmén, Fredrik
Klughammer, Johanna
Stenbeck, Linnea
Schapiro, Denis
Äijö, Tarmo
Bonneau, Richard
Bergenstråhle, Ludvig
Navarro, José Fernandéz
Date Issued
September 2019
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Vickovic, Sanja et al. "High-definition spatial transcriptomics for in situ tissue profiling." Nature Methods 16, 10 (September 2019): 987–990 © 2019 The Author(s)
Version
Author's final manuscript
Abstract
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41592-019-0548-y
