HiCT: High Throughput Protocols For CPE Cloning And Transformation
Name
BBFRFC99.pdf
Size
194.69 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Beer, Ralf
Christiansen, Tania
Herbst, Konrad
Ignatiadis, Nikolaos
Kats, Ilia
Kurzawa, Nils
Meichsner, Johanna
Rabe, Sophie
Riedel, Anja
Sachs, Joshua
Date Issued
October 4, 2013
Series/Report no.
BBF RFC;99
Abstract
The purpose of this RFC is to provide instructions for a rapid and cost efficient cloning and transformation method which allows for the manufacturing of multi-fragment plasmid constructs in a parallelized manner: High Throughput Circular Extension Cloning and Transformation (HiCT).
Description of construct libraries generated by the HiCT method can be found at http://2013.igem.org/Team:Heidelberg/Indigoidine.
This RFC also points out further optimization strategies with regard to construct stability, reduction of transformation background and the generation of competent cells.
Subjects
DNA assembly
Terms of Use
Attribution-NoDerivs 3.0 United States
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