Promoter and Coding Sequence Considerations for Caenorhabditis elegans and Other Eukaryotes
Name
BBF RFC 77.pdf
Size
167.04 KB
Format
Adobe PDF
Checksum (MD5)
d6e890309f8a58e2cf3aec4f7c82ba31
Author(s)
Palmer, Chris
Halliday, Geoff
Date Issued
November 7, 2010
Series/Report no.
BBF RFC;77
Abstract
Our primary aim is to provide a standard format for protein-coding BioBricks intended for
eukaryotes, especially in the nematode worm Caenorhabditis elegans, so that future teams will be
able to correctly take advantage of the Kozak consensus sequence and its power to manipulate
gene expression levels. There are currently, in fact, at least three protein-coding BioBricks with
Kozak sequences that correctly conform to this convention, designed for yeast,
(BBa_K165057, BBa_K165058, and BBa_K165059) but their format has not been
documented or standardized. Since this is so important to full exploitation of current and future
eukaryotic chassis, we feel it must be codified.
Subjects
BBF RFC
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